small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently

A plasmid is a DNA molecule that is separate from the chromosomal DNA and that can replicate (copy itself) independently.[1]

Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids.

The term plasmid was first introduced by the American molecular biologist Joshua Lederberg in 1952.[2]

Plasmids are double stranded and, in many cases, circular. Plasmids usually occur naturally in bacteria, but are sometimes found in eukaryotic organisms (e.g., the 2-micrometre-ring in Saccharomyces cerevisiae).

Plasmid size varies from 1 to over 1,000 kilobase pairs (kbp).[3][4][5] The number of identical plasmids within a single cell can range from one to thousands. Plasmids are often associated with conjugation, a mechanism of horizontal gene transfer.

Plasmids are transferable genetic elements, or "replicons", capable of independent self copying in a suitable host. Plasmids can be found in all three major domains, Archaea, Bacteria and Eukarya.[1] Similar to viruses, plasmids are not considered a form of "life" as it is currently defined.[6] Unlike viruses, plasmids are "naked" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host.

Plasmid host-to-host transfer requires direct, mechanical transfer by conjugation or changes in host gene expression allowing the intentional uptake of the genetic element by transformation.[1]

Microbial transformation with plasmid DNA is neither parasitic nor symbiotic in nature, since each implies the presence of an independent species living in a commensal or detrimental state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and may provide a selective advantage under a given environmental state.

Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or alternatively the proteins produced may act as toxins under similar circumstances. Plasmids also can provide bacteria with an ability to fix elemental nitrogen or to degrade difficult organic compounds. This may provide an advantage under conditions of nutrient deprivation.[1]

The construction of first recombinant DNA emerged from linking of a gene encoding antibiotic resistance with a native plasmid.

References change

  1. 1.0 1.1 1.2 1.3 Lipps G, ed. (2008). Plasmids: current research and future trends. Caister Academic Press. ISBN 978-1-904455-35-6.
  2. Lederberg J (1952). "Cell genetics and hereditary symbiosis". Physiol. Rev. 32 (4): 403–430. doi:10.1152/physrev.1952.32.4.403. PMID 13003535.
  3. Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory.
  4. Barnett M.J.; et al. (200). "Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid". PNAS. 98 (17): 9883–9888. doi:10.1073/pnas.161294798. PMC 55547. PMID 11481432.
  5. Finan T.M.; et al. (2001). "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont". Proceedings of the National Academy of Sciences. 98 (17): 9889–9894. doi:10.1073/pnas.161294698. PMC 55548. PMID 11481431.
  6. Sinkovics, J; Harvath J; Horak A. (1998). "The origin and evolution of viruses (a review)". Acta Microbiologica et Immunologica Hungarica. 45 (3–4). Akademiai Kiado: 349–90. ISSN 1217-8950. PMID 9873943.