Transposition can create significant mutations and alter the cell's genome size.
Transposons are only one of several types of mobile genetic elements. Transposons themselves are of two types according to their mechanism, which can be either "copy and paste" (class I) or "cut and paste" (class II).
Class I (Retrotransposons): They copy themselves in two stages, first from DNA to RNA by transcription, then from RNA back to DNA by reverse transcription. The DNA copy is then inserted into the genome in a new position. Retrotransposons behave very similarly to retroviruses, such as HIV.
Class II (DNA transposons): By contrast, the cut-and-paste transposition mechanisms of class II transposons do not involve an RNA intermediate.:284
As causes of diseaseEdit
Transposons are mutagens. They can damage the genome of their host cell in different ways:
- A transposon or a retroposon that inserts itself into a functional gene will most likely disable that gene.
- After a transposon leaves a gene, the resulting gap will probably not be repaired correctly.
- Multiple copies of the same sequence, such as Alu sequences can hinder precise chromosomal pairing during mitosis and meiosis, resulting in unequal crossovers, one of the main reasons for chromosome duplication.
Transposons can carry accessory genes, such as antibiotic resistance genes. They can be used to put a gene into the DNA of an organism. This has been done with fruit flies (Drosophila melanogaster) by putting the transposon into the embryo.
- The first transposons were discovered in maize (Zea mays), by Barbara McClintock in 1948, for which she was awarded a Nobel Prize in 1983. She noticed chromosome mutations caused by these transposons. About 50% of the total genome of maize consists of transposons. The Ac/Ds system McClintock described are class II transposons.
- One family of transposons in the fruit fly Drosophila melanogaster are called P elements. They seem to have first appeared in the species only in the middle of the twentieth century. Within 50 years, they have spread through every population of the species. Artificial P elements are used to insert genes into Drosophila by injecting the embryo.
- The most common form of transposon in humans is the Alu sequence. It is approximately 300 bases long and can be found between 300,000 and a million times in the human genome.
- Mariner-like elements are another prominent class of transposons found in multiple species including humans. The Mariner transposon was first discovered by Jacobson and Hartl in Drosophila. This Class II transposable element is known for its uncanny ability to be transmitted horizontally in many species. There are an estimated 14 thousand copies of Mariner in the human genome comprising 2.6 million base pairs.
While some transposons may confer benefits on their hosts, most are regarded as selfish DNA parasites. In this way, they are similar to viruses. Various viruses and transposons also share features in their genome structures and biochemical abilities, leading to speculation that they share a common ancestor.
Excessive transposon activity can destroy a genome, which is lethal. Many organisms have developed mechanisms to inhibit them. Bacteria may use gene deletion to remove transposons and viruses from their genomes while eukaryotic organisms use RNA interference (RNAi) to inhibit transposon activity.
In vertebrate animal cells nearly all the 100,000+ DNA transposons in a genome code for inactive polypeptides. In humans, all of the Class I-like transposons are inactive. The first DNA transposon used as a tool for genetic purposes, the Sleeping Beauty transposon system, was a transposon which was resurrected from a long evolutionary sleep.
Role in the immune systemEdit
Transposons may have been co-opted by the vertebrate immune system as a means of producing antibody diversity: The V(D)J recombination system operates by a mechanism similar to that of transposons. This is a system of three genes which get rearranged in the production of vertebrate lymphocytes. The system diversely encode proteins to match antigens from bacteria, viruses, parasites, dysfunctional cells such as tumor cells, and pollens.
The final DNA sequence, and thus the sequence of the antibody, is highly variable, even when the same two V, D, or J segments are joined. This great diversity allows VDJ recombination to generate antibodies even to microbes that neither the organism nor its ancestors have ever previously encountered.
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